Laccase Cross-Linked Ultraporous Aluminas for Sustainable Biodegradation of Remazol Brilliant Blue R

Over the past few decades, enzyme-based green and sustainable chemistry has attracted extensive research attention, which provides a promising alternative to conventional treatment methods of recalcitrant micropollutants. However, enzyme denaturation stability loss remain critical challenges for its potential applications in industrial wastewater treatment. In this study, laccase from Trametes versicolor (laccase T.) was cross-linked immobilized by ultraporous alumina (UPA) biodegradation Remazol Brilliant Blue R (RBBR). Through sequential use an aminosilane coupling agent (3-aminopropyl)triethoxysilane (APTES) bifunctional cross-linker glutaraldehyde (GA), synthesized biocatalysts showed better immobilization performances (about 4-fold physical adsorption). The GA concentration considerably affected T. cross-linking degree, while post-treatment protocol highest yield with lower activity recovery. Moreover, biocatalyst stabilities including pH stability, thermal storage reusability were also studied. Tolerance broader temperature ranges, good UPA(γ) biocatalysts, their continuous RBRR efficiency highlight potentials inorganic materials treatment, can broaden our understanding practical environmental fields.